PRETREATMENT SOLUTION FOR IMMUNOHISTOCHEMICAL STAINING AND CONDENSED SOLUTION THEREOF

摘要

Disclosed are a pretreatment solution for immunohistochemical staining, which elutes a paraffin-containing embedding medium from a glass slide with a tissue specimen embedded in the medium, and retrieves antigenicity of the tissue specimen, and which is usable three or more times, and a pretreatment solution concentrate for immunohistochemical staining which allows ready preparation of the pretreatment solution and a method of immunohistochemical staining using the same. The pretreatment solution contains an antigen retrieving agent, particular nonionic surfactants, and cyclodextrin or a derivative thereof, with the balance being not less than 80 mass % of water. The content of the antigen retrieval agent is such that the pH of the pretreatment solution is in a predetermined range, and the content of cyclodextrin or a derivative thereof is a particular amount.

主权项

1. A method for immunohistochemical staining of a paraffin-embedded tissue sample, which comprises:
a step of simultaneous deparaffinization and antigen retrieval pretreatment, comprising contacting the paraffin-embedded tissue sample with a pretreatment solution of a pH of 5.0-10.0; a step of washing the deparaffinized and antigen-retrieved tissue specimen with said pretreatment solution; and a step of immunohistochemical staining the washed tissue sample, wherein said pretreatment solution comprises an antigen retrieval agent which enables retrieval of antigenicity by heat treatment, two nonionic surfactants, and cyclodextrin or a derivative thereof, with the balance being not less than 80 mass % of water, wherein a content of said antigen retrieval agent is such that a pH of said pretreatment solution is 5.0 to 10.0, said two nonionic surfactants are a polyoxyethylene alkyl phenyl ether nonionic surfactant and a polyoxyethylene sorbitan nonionic surfactant, a content of said polyoxyethylene alkyl phenyl ether nonionic surfactant is 0.01 to 1.0 mass %, a content of said polyoxyethylene sorbitan nonionic surfactant is 0.3 to 3.0 mass %, and a content of said cyclodextrin or a derivative thereof is 0.01 to 1.0 mass %.