| 摘要 |
The invention provides scaffolds, methods of culturing cells on and/or within scaffolds, and methods of making scaffolds, wherein the scaffolding comprises thermoresponsive polymers, e.g., N-isopropylacrylamide (NIPAAm). The scaffolds may include other polymers, cell adhesion factors, polymers that are not NIPAAm, and/or cells. The scaffold also exhibits a phase transition in solution above a lower critical solution temperature (LCST). The scaffolds form structures such as beads and/or threads. The scaffolds can be incorporated into tissue cultures vessels. Also provided are methods for recovering cells cultured on the scaffolds by lowering the temperature below an LCST. |
