NOVEL ATTENUATED POLIOVIRUS: PV-1 MONO-CRE-X

摘要

A novel and stable attenuated poliovirus is produced by engineering an indigenous replication element (cre), into the 5′non-translated genomic region (with inactivation of the native ere element located in the coding region of 2C (mono-crePV), and replacing the nucleic acid sequence of all or part of the capsid coding region (PI) with a substitute PI coding region having reduced codon pair bias. The stably attenuated poliovirus is effective for vaccines and immunization.