Binding and functional assays that use the T1R3 receptor to screen for taste modulatory compounds

摘要

Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.

主权项

1. An in vitro method for identifying a compound that specifically binds to a human T1R3 extracellular domain polypeptide, the method comprising:
(1) contacting a compound with a human T1R3 extracellular domain polypeptide, wherein said T1R3 extracellular domain polypeptide possesses at least 95% sequence identity to amino acid residues 1 to 563 of SEQ ID NO: 4; and (2) detecting whether said compound specifically binds to said T1R 3 extracellular domain polypeptide, thereby determining whether said compound binds to said T1R3 extracellular domain.