Lipid therapy

摘要

A method including introducing into a blood stream a delipidated high density lipoprotein (HDL) and a bioactive agent. A composition including a delipidated high density lipoprotein (HDL) and an auxiliary agent in a form suitable for delivery into a blood vessel. A composition including Apo A1 comprising a hydrophobic ligand suitable to interact with cell surface binding sites. A composition including Apo A1 and an agent selected to one of increase the ATP-binding cassette protein 1 (ABCA1) transporter expression in macrophages and protect ABCA1 from thiol-mediated degradation.

主权项

1. A method for treating a subject comprising:
withdrawing an amount of blood from the subject using a catheter assembly; delipidating high density lipoprotein (HDL) present in the amount of blood, wherein delipidating comprises combining the amount of blood with a solvent and a surfactant; adding a surface coating to the delipidated HDL for increasing a partition coefficient of the delipidated HDL; and after delipidating, (1) combining an exogenous bioactive agent having a hydrophobic ligand bound thereto with the delipidated HDL with the surface coating, wherein the exogenous bioactive agent includes at least one of an apolipoprotein A1 (Apo A1), a functional Apo A1 mutant, and a functional Apo A1 mimetic peptide, and (2) forming a composition comprising (i) the delipidated HDL with the surface coating combined with the exogenous bioactive agent having a hydrophobic ligand bound thereto, and (ii) a viscosifier including at least one of hyaluronic acid, polyvinylpyrrolidone (PVP), a hydroxypropylmethacrylate (HPMA) copolymer system, and carboxymethyl cellulose (CMC); and introducing the composition into a blood stream or tissue of the subject, using the catheter assembly.