PREPARATION OF BEE VENOM REMOVED ALLERGIC INGREDIENTS

摘要

The present invention relates to a method for producing an isolated and purified bee venom without allergenic ingredients to produce the isolated and purified bee venom having a medicinal effect while basically preventing allergy induction by reducing or removing the allergenic ingredients in the bee venom using ultrafiltration. The method comprises: a first step for preparing the purified bee venom powder; a second step for making a bee venom solution by mixing the purified bee venom powder and distilled water; a third step for removing the allergenic ingredients contained in the bee venom solution by filtering the bee venom solution through an ultrafiltration membrane with cut-off size of 10 kDa; and a fourth step for obtaining the isolated and purified bee venom without the allergenic ingredients. At the second step, 1000-1500 ml of the distilled water is mixed with 1 g of the purified bee venom powder. At the third step, the allergenic ingredients removed through the ultrafiltration membrane are phospholipase A2 and hyaluronidase. The isolated and purified bee venom obtained at the fourth step contains at least 4% of apamin and at least 50% of melittin. [Reference numerals] (AA) START; (BB) END; (S11) Preparing purified bee venom powder; (S12) Making a bee venom solution by mixing the purified bee venom powder and distilled water; (S13) Removing the allergenic ingredients contained in the bee venom solution by filtering the bee venom solution through an ultrafiltration membrane with cut-off size of 10 kDa; (S14) Obtaining the isolated and purified bee venom without the allergenic ingredients