| 摘要 |
The invention relates to a method for producing a DNA sequence for a nucleic acid library having a plurality of elements. The synthesized DNA sequence comprises at least three regions. The first region and the third region in each element each have defined sequences (D1, D2) that are identical in each element of the nucleic acid library. In a middle second region, at least two adjacent variable codon triplets are arranged. The following method steps are carried out: First, oligonucleotides (O1-x) of a first oligonucleotide library (O1b) are hybridized with a first largely complementary oligonucleotide (O2) to form a first double-stranded DNA library (O1xO2). Furthermore, oligonucleotides (O3-x) of a second oligonucleotide library (O3b) are hybridized with a second largely complementary oligonucleotide (O4) to form a second double-stranded DNA library (O3xO4). Then the first double-stranded DNA library (O1xO2) and the second double-stranded DNA library (O3xO4) are ligated to form a double-stranded DNA ligation product (LPx). The double-stranded DNA ligation product comprises the following sub-regions: a defined first region (D1) of the nucleic acid library, at least one first variable codon triplet (Tv1), a spacer sequence (S), at least one second variable codon triplet (Tv2), and a defined second region (D2) of the nucleic acid library. After the spacer sequence (S) has been removed, a library of double-stranded DNA product (Px) comprising the following sub-regions is obtained: a defined first region (D1) of the nucleic acid library, at least one first variable codon triplet (Tv1), at least one second variable codon triplet (Tv2), and a defined second region (D2) of the nucleic acid library. |
