DEVELOPMENT OF NUCLEIC ACID GEL MATRIX FOR CELL-FREE PROTEIN SYNTHESIS OF CELL NUCLEUS REPLICATE, AND METHOD FOR PRODUCING SAME

摘要

The present invention relates to a nucleic acid gel matrix for the cell-free protein synthesis of a cell nucleus replicate which contains an X-type nucleic acid nanostructure, an expression plasmid containing a DNA fragment, transcription and translation constituents, and a lipid membrane component, and to a method for producing same. According to the present invention, the nucleic acid gel matrix for the cell-free synthesis of a cell nucleus replicate is a polymorphic (for example, oval, spherical, or rectangular) gel matrix which contains the X-type nucleic acid nanostructure, the expression plasmid containing the DNA fragment, the transcription and translation constituents, and the lipid membrane component. Owing to a (oval) structure which replicates a physical model of a chromosome in the cell nucleus, the DNA and the transcription and translation component factors are gathered in a small space so as to maximize the amount of protein produced. Also, since the DNA is contained in a gel form, protection from external factors is provided so that the DNA can be used to produce high-density protein having stability for a long period of time.