发明名称 |
METHODS FOR MAKING SACCHARIDE-PROTEIN GLYCOCONJUGATES |
摘要 |
The invention provides a process for the reductive amination of a carbonyl group at the reducing terminus of a polysaccharide, wherein the reductive amination is carried out at a pH between 4 and 5. The invention also provides a process for preparing a conjugate of a polysaccharide and a carrier molecule, comprising the steps of: (a) coupling the polysaccharide to a linker, to form a polysaccharide-linker compound in which the free terminus of the linker is an ester group; and (b) reacting the ester group with a primary amine group in the carrier molecule, to form a polysaccharide-linker-carrier molecule conjugate in which the linker is coupled to the carrier molecule via an amide linkage. The invention also provides a process for reducing contamination of a polysaccharide-linker compound with unreacted linker, comprising a step of precipitating unreacted linker under aqueous conditions at a pH of less than 5. The invention also provides polysaccharide-linker-carrier molecule conjugates and intermediate compounds obtained or obtainable by these processes. |
申请公布号 |
WO2013038375(A2) |
申请公布日期 |
2013.03.21 |
申请号 |
WO2012IB54805 |
申请日期 |
2012.09.14 |
申请人 |
NOVARTIS AG;SAUL, ALLAN;MICOLI, FRANCESCA |
发明人 |
SAUL, ALLAN;MICOLI, FRANCESCA |
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