发明名称 |
DIFFERENTIATION AND PROLIFERATION METHOD FOR NATURAL KILLER CELLS FROM CD14-POSITIVE MONOCYTES |
摘要 |
<p>The present invention relates to an effective differentiation and proliferation method for natural killer cells (NK cells) from cord blood, and more specifically, to a method for effectively differentiating and proliferating natural killer cells from cord blood, comprising the following steps: 1) removing CD3, CD34, CD56 and CD19 positive cells from cord blood-derived monocytes to prepare CD3-CD34-CD56-CD19 cells; 2) removing CD14-negative cells from the CD3-CD34-CD56-CD19 cells to prepare CD3-CD34-CD56-CD19-CD14-positive cells; and 3) mixing the CD3-CD34-CD56-CD19-CD14-positive cells with IL-15 and IL-21 and culturing the mixture. The present invention can obtain NK cells with a high purity within a short time by inducing NK cells from the CD14-positive cells, compared with a method inducing NK cells from hematopoietic stem cells. In addition, the present invention can induce NK cells with an excellent cell killing activity within a short time so as to differentiate the same into NK cells with a cancer cell killing properties, and thus can be useful for treatment of cancer cells.</p> |
申请公布号 |
WO2012118349(A9) |
申请公布日期 |
2012.10.11 |
申请号 |
WO2012KR01587 |
申请日期 |
2012.03.02 |
申请人 |
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY;CHOI, IN PYO;YOON, SUK RAN;KIM, MI-JOUNG |
发明人 |
CHOI, IN PYO;YOON, SUK RAN;KIM, MI-JOUNG |
分类号 |
C12N5/0783;A61K35/12;A61P35/00;C07K14/54 |
主分类号 |
C12N5/0783 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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