Provided is an analytical method which enables accurate quantitative determination of each vitamin E homolog in an oil or fat containing tocopherol and tocotrienol. Each vitamin E homolog in an oil or fat containing tocopherol and tocotrienol can be quantitatively determined accurately by high-performance liquid chromatography (HPLC) using a normal phase column as a separation column, and using hexane : ethyl acetate : acetic acid = 98.5 to 99.8 : 0.1 to 0.5 : 0.1 to 1 (v/v) as a mobile phase.