| 摘要 |
PURPOSE: A TAIL PCR method for analyzing mutant genome generated by insertion of a foreign gene in Brassicaceae genus plant is provided to quickly analyze a small amount of DNA. CONSTITUTION: A PCR analysis method for analyzing mutant genome generated by insertion of a foreign gene Brassicaceae genus plant comprises: a first TAIL(thermal asymmetric interlaced) PCR reaction using an arbitrary degenerate primer selected from sequence numbers 1-6 and a first specific primer; a second TAIL PCR reaction using an arbitrary degenerate primer and second specific primer; and third TAIL PCR reaction using an arbitrary degenerate primer and third specific primer. |
