发明名称 |
METHODS AND COMPOSITIONS FOR A SOYBEAN IN-PLANTA TRANSIENT EXPRESSION SYSTEM |
摘要 |
The present invention provides methods of transiently expressing a nucleotide sequence in one or more cells of a soybean plant, comprising: a) abrading the surface of a soybean plant and/or intact part thereof; and either (b1) immersing the abraded soybean plant and/or abraded intact part thereof in a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nucleotide sequence to be transiently expressed; (b2) applying a vacuum to the immersed soybean plant and/or intact part thereof of step (b1); and (b3) releasing the vacuum, thereby introducing the solution into the soybean plant and/or intact part thereof; or (c) contacting the abraded soybean plant and/or abraded intact part thereof with a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nucleotide sequence to be transiently expressed, whereby the nucleotide sequence is transiently expressed in one or more cells of the soybean plant. |
申请公布号 |
WO2012091939(A1) |
申请公布日期 |
2012.07.05 |
申请号 |
WO2011US65092 |
申请日期 |
2011.12.15 |
申请人 |
SYNGENTA PARTICIPATIONS AG;AZHAKANANDAM, KASIMALAI;SU, PEI |
发明人 |
AZHAKANANDAM, KASIMALAI;SU, PEI |
分类号 |
A01H1/00 |
主分类号 |
A01H1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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