A group of esterases for the enantioselective production of fine and speciality chemicals

摘要

The present invention relates to a polynucleotide consisting of more than 30 nucleotides encoding an enzyme having carboxylesterase [E.C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in SEQ ID NO: 6; (b) a polynucleotide consisting of or comprising a nucleotide sequence, wherein the nucleic acid sequence is as shown in SEQ ID NO: 5; (c) a polynucleotide consisting of or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one amino acid residue is conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxylesterase activity which polynucleotide is at least 66% identical to a polynucleotide encoding an enzyme as shown in SEQ ID NO: 5; (e) a polynucleotide consisting of or comprising a nucleotide sequence the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined in any one of (a) to (b); and (f) a polynucleotide consisting of or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (e); or the complementary strand of such a polynucleotide of (a) to (f).