发明名称 |
UNIVERSAL NUCLEIC ACID PROBE SET AND METHOD FOR UTILIZATION THEREOF |
摘要 |
<p>A nucleic acid probe set includes (A) a fluorescent probe and (B) a binding probe. The fluorescent probe (A) is formed of an oligonucleotide, which includes (a) a nucleotide unit labeled with (d) a fluorescent substance. The binding probe (B) is formed of an oligonucleotide having (b1) a fluorescent probe binding region, which can hybridize to the fluorescent probe (A), and (b2) a target nucleic acid binding region, which can hybridize to a target nucleic acid sequence (C). The fluorescent substance (d) is a fluorescent substance which changes in fluorescent character upon interaction with guanine. At least one of nucleotide units which constitute the fluorescent probe (A) is an artificial nucleotide unit having a function to raise a dissociation temperature between the probe (A) and the fluorescent probe binding region (b1). The nucleic acid probe is provided with an improved fluorescence quenching efficiency.</p> |
申请公布号 |
EP2333059(A1) |
申请公布日期 |
2011.06.15 |
申请号 |
EP20090803013 |
申请日期 |
2009.07.30 |
申请人 |
NIPPON STEEL KANKYO ENGINEERING CO., LTD. |
发明人 |
SEKIGUCHI YUJI;NODA NAOHIRO;MIYATA RYO;NAKAMURA KAZUNORI;KURATA SHINYA;TSUNEDA SATOSHI;TANI HIDENORI |
分类号 |
C12Q1/68;C12N15/09 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|