Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; and correlating a signal of the label with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample.
申请公布号
DE60143960(D1)
申请公布日期
2011.03.10
申请号
DE2001643960
申请日期
2001.05.18
申请人
ERAGEN BIOSCIENCES INC.
发明人
MARSHALL, DAVID J.;PRUDENT, JAMES R.;SCHERRILL, CHRISTOPHER B.;SHAPIRO, GIDEON;GRENIER, JENNIFER K.;RICHMOND, CRAIG S.;JURCZYK, SIMONA;PTACIN, JEROD L.