HYPO-IMMUNOGENIC STREPTAVIDIN AND USE THEREOF

摘要

Disclosed is a streptavidin mutant in which the immunogenicity (antigenicity) of streptavidin in mammals has been reduced. The core streptavidin amino acid sequence of the streptavidin mutant, described by sequence no. 2, contains an amino acid sequence in which (a) the arginine at amino acid residue 72 is replaced with another amino acid, and (b) any one or more of the tyrosine at amino acid residue 10, tyrosine at amino acid residue 71, glutamic acid at amino acid residue 89, arginine at amino acid residue 91, and glutamic acid at amino acid residue 104 is replaced with another amino acid, thereby reducing the immunogenicity compared to wild-type streptavidin.