发明名称 |
METHOD OF STABLE CELL CULTURES MANUFACTURE |
摘要 |
FIELD: medicine. ^ SUBSTANCE: embryonic tissue is flushed with enzyme solution at 25-37C temperature. For enzyme neutralisation is used medium, made of Eagle medium with lactalbumine hydrolysate or bovine serum. ^ EFFECT: due to reducing of trypsin proteolitic activity and higher development temperature in stable cell cultures manufacture, yield of viable diploid cells is increased in 1,5-2 times compared to closest analogue of this invention. ^ 7 cl, 4 tbl |
申请公布号 |
RU2392318(C1) |
申请公布日期 |
2010.06.20 |
申请号 |
RU20080145261 |
申请日期 |
2008.11.17 |
申请人 |
FEDERAL'NOE GOSUDARSTVENNOE UCHREZHDENIE NAUKI "EKATERINBURGSKIJ NAUCHNO-ISSLEDOVATEL'SKIJ INSTITUTVIRUSNYKH INFEKTSIJ" FEDERAL'NOJ SLUZHBY PO NADZORU V SFERE ZASHCHITY PRAV POTREBITELEJ I BLAGOPOLUCHIJA CHELOVEKA |
发明人 |
GLINSKIKH NINA POLIKARPOVNA;BAKHAREV ALEKSEJ ANATOL'EVICH;UST'JANTSEV PAVEL VASIL'EVICH;UST'JANTSEV IGOR' VASIL'EVICH |
分类号 |
A61K39/00;C12N5/07;C12N9/76 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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