发明名称 METHOD OF STABLE CELL CULTURES MANUFACTURE
摘要 FIELD: medicine. ^ SUBSTANCE: embryonic tissue is flushed with enzyme solution at 25-37C temperature. For enzyme neutralisation is used medium, made of Eagle medium with lactalbumine hydrolysate or bovine serum. ^ EFFECT: due to reducing of trypsin proteolitic activity and higher development temperature in stable cell cultures manufacture, yield of viable diploid cells is increased in 1,5-2 times compared to closest analogue of this invention. ^ 7 cl, 4 tbl
申请公布号 RU2392318(C1) 申请公布日期 2010.06.20
申请号 RU20080145261 申请日期 2008.11.17
申请人 FEDERAL'NOE GOSUDARSTVENNOE UCHREZHDENIE NAUKI "EKATERINBURGSKIJ NAUCHNO-ISSLEDOVATEL'SKIJ INSTITUTVIRUSNYKH INFEKTSIJ" FEDERAL'NOJ SLUZHBY PO NADZORU V SFERE ZASHCHITY PRAV POTREBITELEJ I BLAGOPOLUCHIJA CHELOVEKA 发明人 GLINSKIKH NINA POLIKARPOVNA;BAKHAREV ALEKSEJ ANATOL'EVICH;UST'JANTSEV PAVEL VASIL'EVICH;UST'JANTSEV IGOR' VASIL'EVICH
分类号 A61K39/00;C12N5/07;C12N9/76 主分类号 A61K39/00
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