COMBINATION MICROSCOPY

摘要

The invention relates to a method for generating an image of a sample by means of a microscopy method comprising varying local resolution, wherein at least two of the following microscopy methods are combined: laser scanning microscopy for generating an LSM microscopy image, a microscopy method wherein the sample is excited to luminescence by structured line or wide area illumination, the structure is rotated and displaced several times for each rotary position, wherein at least three rotary positions and three displacement positions per rotary position are implemented, the luminescent sample is imaged on a surface detector for each position, and a first microscopy image is generated from the images thus obtained, having increased local resolution greater than the optical resolution of the image, a further microscopy method according to the PAL principle, by means of which a second microscopy image is generated, indicating geometric locations of marker molecules emitting luminescent radiation at an increased local resolution relative to the optical resolution, and a further microscopy method, wherein the sample is marked using marking molecules suitable for the STED, ESA, or RESOLFT technique, and a third microscopy image is generated by means of STED, ESA, or RSEOLFT, wherein the obtained microscopy images are superimposed.