METHOD FOR CONSTRUCTING A VIRAL GENOMIC LIBRARY FROM THE STATE IN WHICH VIRUSES ARE MIXED WITH THE INFECTED HOSTS

摘要

There is provided a method for constructing a fosmid library of Pieris rapae, which comprises the steps of: separating genomic DNA from a Pieris rapae virus and Pieris rapae; washing the genomic DNA; preparing genomic DNA embedded agarose plugs; performing electrophoresis and dialysis on the agarose plugs; partially digesting the agarose plugs; separating DNA of the Pieris rapae virus from DNA of the Pieris rapae; mapping fosmid clones of the separated DNA of the Pieris rapae virus; and shotgun library construction of the mapped fosmid clones.