发明名称 |
Nonhuman model animal unresponsive to immunopotentiating synthetic compound |
摘要 |
The present invention relates to provide a non-human animal model unresponsive to a synthetic compound wherein a gene function encoding TLR7 that recognizes an immunopotentiating synthetic compound such as imidazoquinoline lacks on is genomic locus. Whole or part of a gene fragment of a gene site including an intracellular region and a transmembrane region of a TLR7 gene obtained from a mouse gene library is replaced by a plasmid including poly A signal and a marker gene to construct a targeting vector. Then, this targeting vector is linearized and transferred into embryonic stem cells. The target embryonic stem cells wherein the TLR7 gene function is deleted are microinjected into a mouse blastocyst to generate a chimeric mouse. Then, this chimeric mouse is crossed with a wild-type mouse to generate a heterozygote mouse. Next, the heterozygote mice are intercrossed to obtain a TLR7 knockout mouse.
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申请公布号 |
US7608750(B2) |
申请公布日期 |
2009.10.27 |
申请号 |
US20040496501 |
申请日期 |
2004.07.28 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY AGENCY;DAINIPPON SUMITOMO PHARMA CO., LTD. |
发明人 |
AKIRA SHIZUO;TOMIZAWA HIDEYUKI;YAMAOKA TAKASHI |
分类号 |
A01K67/00;A01K67/027;A61P37/04;C07K14/705;C12N15/00;C12N15/85 |
主分类号 |
A01K67/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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