发明名称 |
Soluble rubella E1 envelope protein variants |
摘要 |
A soluble rubella E1 antigen variant is disclosed that comprises amino acids 334-409 of the native rubella E1 peptide, but lacks the C-terminal end and at least the transmembrane region and the anchor segment as well as at least the amino acids 143 to 164. Also described is a recombinant DNA molecule encoding the rubella E1 antigen variants which are recombinantly expressed as a chaperone fusion protein, refolded into a soluble and immunoreactive conformation, and further used for the serological detection of anti-rubella antibodies. In addition, also disclosed is a method for the detection, determination and quantification of anti-rubella antibodies of IgG and/or IgM subclass in a sample wherein the rubella E1 antigen is used as a capture reagent and/or binding partner for the antibodies.
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申请公布号 |
US7604935(B2) |
申请公布日期 |
2009.10.20 |
申请号 |
US20060584887 |
申请日期 |
2006.10.23 |
申请人 |
ROCHE DIAGNOSTICS OPERATIONS, INC. |
发明人 |
UPMEIER BARBARA;BOLLHAGEN RALF;ENGEL ALFRED;FAATZ ELKE;SCHAARSCHMIDT PETER;SCHOLZ CHRISTIAN;ZARNT TORALF |
分类号 |
C12Q1/70;A61K39/20;C12N7/01 |
主分类号 |
C12Q1/70 |
代理机构 |
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