发明名称 |
METHOD AND COMPOSITION FOR POLYPEPTIDE ENGINEERING |
摘要 |
<P>PROBLEM TO BE SOLVED: To provide a number of strategies for evolving a polypeptide through a recursive recombination method. <P>SOLUTION: A method for evolving a protein encoded by a DNA substrate molecule contains the following steps: (a) a step to digest at least the first and the second DNA substrate molecules with a restriction endonuclease, wherein the first and the second substrate molecules are different from each other at least by one nucleotide; (b) a step to connect the mixture for producing a library of recombinant DNA molecules; (c) a step to screen or select the product of the step (b) by a desired characteristic; and (d) a step to recover the recombinant DNA substrate molecule encoding the evolved protein. <P>COPYRIGHT: (C)2009,JPO&INPIT |
申请公布号 |
JP2009089710(A) |
申请公布日期 |
2009.04.30 |
申请号 |
JP20080306897 |
申请日期 |
2008.12.01 |
申请人 |
MAXYGEN INC |
发明人 |
PATTEN PHILLIP A;STEMMER WILLEM P C |
分类号 |
C12N15/09;A61K38/45;A61K48/00;C07K14/005;C07K14/435;C07K14/47;C07K14/545;C07K14/56;C07K16/00;C07K19/00;C12N7/00;C12N9/02;C12N9/10;C12N9/12;C12N9/38;C12N9/86;C12N15/10;C12N15/52;C12N15/64;C12P21/02;C12Q1/02;C12Q1/37;C12Q1/68;C40B40/02;G01N33/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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