| 摘要 |
A method for creating networks of perfusable microvessels (222) in vitro. A mandrel (916) is drawn through a matrix (1054) to form a channel through the matrix (1010). Cells (1) are injected into the channel (1014). The matrix (1054) is incubated to allow the cells (1) to attach inside the channel (1016). The channel is perfused (1020) to remove unattached cells to create a parent vessel (1022), where the parent vessel (1102) includes a perfusable hollow channel lined with cells (1) in the matrix (1054). The parent vessel (1102) is induced to create sprouts (1106) into the surrounding matrix gel (1054) so as to form a microvessel network (222). The microvessel network (222) is subjected to luminal perfusion through the parent vessel (1102). |
