发明名称 |
METHODS OF USING NUCLEOTIDE INTEGRASE FOR CLEAVING DNA AND ATTACHING NUCLEIC ACIDS |
摘要 |
The present invention provides new methods, employing a nucleotide integrase , for cleaving double-stranded and single stranded DNA substrates at specific sites and for attaching nucleic acid molecules to the cleaved DNA substrates. One method uses a nucleotide integrase to cleave one strand of a double-stranded DNA and to concomitantly attach a nucleic acid molecule to the cleaved strand. Another method uses a nucleotide integrase to cleave both strands of a double-strand ed DNA substrate and to attach a nucleic acid molecule to one strand of the DNA substrate. Another method uses a nucleotide integrase to cleave both strands of a double-stranded DNA substrate and to attach an RNA molecule to one strand of the substrate and for attaching a cDNA to the other strand of the substrate. Another method cleaves single stranded DNA with the concomitant insertion of a nucleic acid molecule at the cleavage point. The nucleotide integrase comprises an RNP particle which comprises a group II intron RNA bound to a group II intron encoded protein. The present invention also relates to purified and reconstituted RNP particles and reconstituted RNP th at cleave DNA substrates.
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申请公布号 |
CA2229075(C) |
申请公布日期 |
2009.02.03 |
申请号 |
CA19962229075 |
申请日期 |
1996.09.11 |
申请人 |
THE OHIO STATE RESEARCH FOUNDATION |
发明人 |
ZIMMERLY, STEVEN;LAMBOWITZ, ALAN M.;YANG, JIAN;GUO, HUATAO |
分类号 |
C12N9/22;C12N15/09;C12N9/00;C12N9/16;C12N15/10;C12N15/66;C12P19/34;C12Q1/68 |
主分类号 |
C12N9/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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