| 摘要 |
PURPOSE:To produce the titled substance easily in high yield in a short time without using a preliminary reactor, by supplying a part of a reagent necessary for the condensation to a channel connected to a reactor, passing raw materials through the channel, finally passing the remaining part of the reagent through the channel, and charging the reagent together with the raw materials in the channel to the reactor. CONSTITUTION:Condensation reaction is carried out using a synthesizing apparatus constructed to feed the reagents and raw materials necessary for a polynucleotide synthesis reaction to a reactor at a predetermined order and discharging the reaction products from the reactor. In the above procedure, the reactor is charged with a solvent from the solvent bottle 2, a detritylation agent from the reagent bottle 3, an oxidizing agent from the reagent bottle 4, a capping agent from the reagent bottle 5 and an activation agent from the reagent bottle 6, and a part of the reagents necessary for the condensation reaction is passed through the channel 14b connected to the reactor. Raw materialss such as adenine, guanine, cytosine, thymine, etc., are supplied from the raw material bottles 7-10 to the channel 14b in predetermained order, and finally the remaining part of the reagents are passed through the channel and supplied together with the raw materials in the channel to the reactor 11 to effect the reaction of the components.
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