发明名称 |
SOLID PHASE FOR CAPTURE OF NUCLEIC ACIDS |
摘要 |
A method of: providing a solid surface having a dendrimer molecule bound thereto and a single-stranded probe nucleic acid immobilized to the dendrimer; contacting the solid surface with a sample suspected or known to contain a double-stranded complimentary target nucleic acid; denaturing the target nucleic acids at thermal conditions and in a salt concentration sufficient to denature the target nucleic acids to produce denatured nucleic acids; and cooling the sample to allow hybridization of the denatured nucleic acids to the probe nucleic acids. An article having: one or more paramagnetic microbeads; a dendrimer molecule bound to the beads; and a probe nucleic acid immobilized to the dendrimer.
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申请公布号 |
US2008293594(A1) |
申请公布日期 |
2008.11.27 |
申请号 |
US20070751096 |
申请日期 |
2007.05.21 |
申请人 |
THE GOVERMMENT OF THE US, AS REPRESENTED BY THE SECRETARY OF THE NAVY |
发明人 |
ARCHER MARIE J.;LIN BAOCHUAN;STENGER DAVID A. |
分类号 |
C40B40/08;C40B50/18 |
主分类号 |
C40B40/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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