发明名称 METHOD AND DEVICE FOR PREPARING SAMPLE
摘要 PROBLEM TO BE SOLVED: To provide a method and a device for simultaneously and individually amplifying, taking out and analyzing a plurality of kinds of nucleic acids (cDNA, mRNA) in samples. SOLUTION: A method which comprises fractionating to micro-droplets of M pieces more than total number N of DNA molecules, a sample solution containing in gel micro-droplets 4, 5, 7, one or less DNA molecule 1-3 to be analyzed, amplifying the DNA in each droplet, in an emulsion 11 containing the droplets by PCR, and, thereafter, detecting existence (amount) of the amplified products obtained in each micro-droplet by fluorometry using an intercalator or the like, and devices therefor are provided. COPYRIGHT: (C)2009,JPO&INPIT
申请公布号 JP2008245612(A) 申请公布日期 2008.10.16
申请号 JP20070093618 申请日期 2007.03.30
申请人 HITACHI LTD 发明人 MURAKAWA KATSUJI;TAKIGUCHI SUMIYO;KANBARA HIDEKI
分类号 C12Q1/68;C12M1/00;C12N15/09 主分类号 C12Q1/68
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