发明名称 |
AAV VECTORS WITH IMPROVED REP CODING SEQUENCES FOR PRODUCTION IN INSECT CELLS |
摘要 |
The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the c ells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins. |
申请公布号 |
CA2921594(A1) |
申请公布日期 |
2007.12.27 |
申请号 |
CA20072921594 |
申请日期 |
2007.06.20 |
申请人 |
UNIQURE IP B.V. |
发明人 |
HERMENS, WILHELMUS THEODORUS JOHANNES MARIA CHRISTIAAN;HAAST, SASKIA JACOBA PETRONELLA;BIESMANS, DENNIS JOHAN;BAKKER, ANDREW CHRISTIAN |
分类号 |
C12N15/35;C07K14/015;C12N5/10;C12N7/01;C12N15/864;C12N15/866 |
主分类号 |
C12N15/35 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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