发明名称 |
Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification |
摘要 |
An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.
|
申请公布号 |
US2006177842(A1) |
申请公布日期 |
2006.08.10 |
申请号 |
US20050252506 |
申请日期 |
2005.10.17 |
申请人 |
WANGH LAWRENCE J;RICE JOHN;SANCHEZ J A;PIERCE KENNETH;SALK JESSE;REIS ARTHUR;HARTSHORN CRISTINA |
发明人 |
WANGH LAWRENCE J.;RICE JOHN;SANCHEZ J. A.;PIERCE KENNETH;SALK JESSE;REIS ARTHUR;HARTSHORN CRISTINA |
分类号 |
C12Q1/68;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|