New polynucleotide encoding insect BUB-1 kinase domain, useful as a target for identification of insecticides, also new protein, vector and recombinant cells

摘要

Isolated polynucleotide (I) comprising a sequence that encodes a insect BUB-1 kinase domain polypeptide (II) comprising a defined 658 amino acids sequence (SEQ ID NO:2) given in the specification, is new. Independent claims are also included for: (1) isolated polynucleotide (I) comprising a sequence with at least 75% identity with region 94-2067 of sequence (1), reproduced; (2) isolated polynucleotide (Ib) comprising a sequence that hybridizes under stringent conditions to the 94-2067 region of sequence (1); (3) recombinant vector containing (I), (Ia) or (Ib); (4) recombinant host cell that contains the vector of (3); (5) method for preparing an insect BUB-1 kinase domain (III) by culturing cells of (4); (6) purified protein having a sequence at least 80% identical with sequence (2); and (7) method for detecting an agent (X) that reduces the enzymatic activity of (III). ACTIVITY : Insecticide. No biological data given. MECHANISM OF ACTION : Inhibition of: (a) activity of BUB-1 kinase (III); or (b) synthesis of (III) or (III)-encoding mRNA. (III) is involved in the spindel checkpoint and absence of it triggers anaphase in presence of agents that cause microtubular depolymerization.