发明名称 |
METHOD OF PURIFYING MODIFIED ACARIAN MAIN ALLERGEN |
摘要 |
A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.
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申请公布号 |
EP1621615(A1) |
申请公布日期 |
2006.02.01 |
申请号 |
EP20040727416 |
申请日期 |
2004.04.14 |
申请人 |
JURIDICAL FOUNDATION THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE |
发明人 |
KOYANAGI, SATOSHI;KAWATSU, KENJIRO;MURAKAMI, TOSHIO;MIYATSU, YOSHINOBU;MAEDA, TOSHIHIRO;MIZOKAMI, HIROSHI |
分类号 |
A61K35/64;A61P37/08;C07K14/435;(IPC1-7):C12N15/09;A61K38/17 |
主分类号 |
A61K35/64 |
代理机构 |
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