| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for clearly and simply detecting a nucleic acid sequence rapidly and precisely in a good reproducibility without requiring complex operations. SOLUTION: This method for detecting the nucleic acid sequence by using a liquid-passing filter, by performing an amplification reaction by using an oligonucleotide for a specific nucleic acid sequence amplification in a specimen solution containing the specific nucleic acid sequence, at the same time with or after the amplification reaction, bringing the above in contact with a bonded material-bonded with a second ligand capable of specifically bonding with the nucleic acid sequence and detecting the complex bonded with the bonded material comprises a process of bonding the first ligand part of the complex with a liquid-passing filter bonded with a first ligand-capturing agent, a process of bonding a labeled physiologically active substance with the second ligand bonded with the liquid-passing filter through the first ligand-capturing agent and the ligand part and a process of measuring the label bonded with the liquid-passing filter. COPYRIGHT: (C)2006,JPO&NCIPI
|
