Tissue engineered liver constructs

摘要

The invention is directed to methods for producing a decellularized liver or part of an liver. A decellularized organ is produced using an isolated liver mechanically agitated to remove cellular membranes surrounding the isolated liver without destroying the interstitial structure of the liver. After the cellular membrane is removed, the isolated liver is exposed to a solubilizing fluid that extracts cellular material without dissolving the interstitial structure of the liver. A washing fluid is used to remove the solubilized components, leaving behind a decellularized liver.