发明名称 |
НИЗКОТЕМПЕРАТУРНОЕ ЦИКЛИЧЕСКОЕ УДЛИНЕНИЕ ДНК С ВЫСОКОЙ СПЕЦИФИЧНОСТЬЮ ПРАЙМИРОВАНИЯ |
摘要 |
The invention relates to methods for extending a primer or a pair of primers in low-temperature cycle DNA amplification for cycle sequencing and PCR. In particular, the methods contemplate the combined use of moderately thermostable DNA polymerases in the presence of a low concentration of glycerol or ethylene glycol, or the mixtures thereof, as an agent to reduce the melting temperature of DNA (that is, the temperature at which the double-strands of DNA are denatured). Predistributed reaction mixtures of a high-fidelity and high processivity DNA polymerase stable at room temperature for several weeks in ready-to-use kits are also contemplated by the invention. |
申请公布号 |
RU2004100107(A) |
申请公布日期 |
2005.06.10 |
申请号 |
RU20040100107 |
申请日期 |
2002.06.05 |
申请人 |
ШАНХАЙ МЕНДЕЛЬ ДНА СЕНТЕР КО., ЛТД (CN) |
发明人 |
ХУН Го Фань (CN);ЯН Юнцзе (CN);ЧЖУ Цз (CN) |
分类号 |
C12N15/09;C12N15/10;C12P19/34;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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