Method for the inactivating of amylase in the presence of protease

摘要

The invention relates to a method for the inactivation of amylase in a solution comprising Rhizomucor miehei aspartic protease by keeping the solution at a pH between 2.1 and 2.8 and at a temperature between 20° C. and 40° C. for a period of time sufficient to inactivate amylase for at least 95%. With this method the level of residual Rhizomucor miehei aspartic protease activity in the solution at the end of the heating time is at least 90%, preferably at least 95%, and the residual amylase activity is 0.05 RAU/g or lower, more preferably 0.001 RAU/g or lower, i.e. more than 99% of the amylase activity has been inactivated.