Method for quantifying a target nucleic acid

摘要

An object of the present invention is to provide a method for quantifying a target nucleic acid by PCR. The present invention provides a method for quantifying a target nucleic acid by the polymerase chain reaction utilizing a template nucleic acid comprising a sequence of the target nucleic acid, a pair of primers for amplifying the target nucleic acid, and polymerase, wherein the polymerase chain reaction is conducted in the presence of a pair of competitive primers having a sequence complementary to the sequence of the amplification primer.