PLASMID HAVING FUNCTION OF T-VECTOR AND EXPRESSION VECTOR TO EXPRESS THE TARGET PROTEIN THROUGH ONE CLONING STEP, AND EXPRESSION OF TARGET GENE USING THE SAME

摘要

PURPOSE: A plasmid having a function of T-vector and expression vector, and expression of a target gene using the same plasmid are provided, which plasmid is easily converted to T-vector, and allows for expression of the target protein through one cloning step. CONSTITUTION: The plasmid pHCE-ROREX having a function of T-vector and expression vector comprises two recognition sites where the T-vector is able to be cloned downstream of a promoter in a vector pHCE which continuously highly expresses a target protein without limitation by a host cell, wherein the recognition site is selected from HphI, MboII, AspEI and XcmI; and a polynucleotide is inserted between two recognition sites. An expression vector is prepared by digesting the plasmid with the recognition enzyme to remove the polynucleotide inserted, and inserting a target gene into the polynucleotide removed site in the plasmid. The method for expression of the target gene comprises transforming a bacterium with the expression vector containing the target gene, and culturing the transformed bacterium.