The present invention refers to new a salt-resistant thermotolerant glutaminase from Lactobacilli. This glutaminase presents a salt dependence such that its activity in the presence of up to 15% NaCl is from 80 to 180 % of that without NaCl and a thermal stability such that its energy of activation is about 80 to 150 kJ.mol<-1>. This enzyme mas be obtained by cultivating a micro-organism belonging to the genus Lactobacillus, producing the salt-resistant thermotolerant glutaminase and then recovering it. The present glutaminase may be useful for enhancing the yield of production of glutamate in biohydrolysate manufacture process. <IMAGE>
申请公布号
EP1427293(A1)
申请公布日期
2004.06.16
申请号
EP20020777040
申请日期
2002.09.06
申请人
SOCIETE DES PRODUITS NESTLE S.A.
发明人
ZIADE, ALEXANDRA;AFFOLTER, MICHAEL;GERBER DECOMBAZ, CHRISTIANE;JAEGER, DANIEL