Medium for measuring effect of tumor therapy on individual cells, useful for selecting treatments, has low buffering capacity in which acidification caused by metabolism of carbon sources is monitored

摘要

Medium (A) for measuring the effects of a tumor therapy on suspensions of individual cells comprises the essential amino acids, vitamins, salts and carbon sources and, the new features, contains 0.1-1 mM buffer (pH 7-7.4); 2-10 g/l glucose and 2-5 mM glutamine as carbon sources and 5-20vol.% fetal calf serum (FCS). An Independent claim is also included for measuring the effects of a tumor therapy on suspensions of individual cells by determining formation of acid in (A), in presence and absence of a cytostatic or cytotoxic substance.