发明名称 |
METHOD OF PLASTID TRANSFORMATION IN ASTERACEAE, VECTOR FOR USE THEREIN AND PLANTS THUS OBTAINED |
摘要 |
The present invention relates to a method for the transformation of plastid genomes of plant species, in particular Asteraceae plant species, comprising the steps of providing a transformation vector carrying a DNA sequence of interest; subjecting a plant material, which comprises plastids, to a transformation treatment in order to allow the plastids to receive the transformation vector; placing the thus treated plant material for a period of time into contact with a culture medium without selection agent; subsequentl y placing the plant material into contact with a culture medium comprising a selection agent; and refreshing the culture medium comprising a selection agent to allow plant material comprising plastids that have acquired the DNA of interest to grow into transformants. |
申请公布号 |
CA2494986(A1) |
申请公布日期 |
2004.02.26 |
申请号 |
CA20032494986 |
申请日期 |
2003.08.08 |
申请人 |
RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V. |
发明人 |
NUGENT, JACKIE M.;LELIVELT, CECILIA LUCIA CLARA;MCCABE, MATTHEW S. |
分类号 |
C07K14/11;C12N15/82;(IPC1-7):C12N15/82 |
主分类号 |
C07K14/11 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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