| 摘要 |
The present invention relates to a method of analyzing peptide(s). More specifically, the method of analyzing peptide(s) in one or more sample(s) comprises the steps of derivatizating said peptide(s) using a chemical assisted fragmentation reagent to produce sample solution(s) containing a mixture of un-derivatised peptides and one or more derivatives; flowing said sample solution(s) through an ion exchange medium and eluting said sample(s) without organic solvents, whereby un-derivatised peptides are captured by said medium and derivatives pass through said medium; and analyzing at least one said derivative in the sample solution outputted from said ion exchange medium. Preferably RPC and MS is run after the ion exchange step. |
