发明名称 |
METHODS OF DETECTING SEQUENCE DIFFERENCES |
摘要 |
The invention relates to methods of genotyping single nucleotide differences in a nucleic acid sample. More particularly, the invention provides methods of identifying the nucleotide at a polymorphic site or a group of polymorphic sites in a sample of genomic DNA. The method uses tagged primer extension in which a set of tag sequences correspond to the identity of the nucleotides at the polymorphic sites. Primer extension products are PCR amplified using a common set of tag-specific primers, the downstream primers bearing distinguishable labels. Following separation by size and/or charge, the detection of distinguishable label in a product of the anticipated size determines the identity of the nucleotide at the polymorphic site. The method is well-suited for the genotyping of multiple single-nucleotide differences in one series of reactions. |
申请公布号 |
WO2004003136(A2) |
申请公布日期 |
2004.01.08 |
申请号 |
WO2003US19576 |
申请日期 |
2003.06.20 |
申请人 |
SENTION, INC.;SLEPNEV, VLADIMIR, I. |
发明人 |
SLEPNEV, VLADIMIR, I. |
分类号 |
G01N21/78;C07H21/04;C12N;C12N15/09;C12P19/34;C12Q1/68;G01N27/447 |
主分类号 |
G01N21/78 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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