发明名称 |
Method of electrophoresis |
摘要 |
The present invention relates a method of electrophoretic separation of protein and/or peptide components of a sample in a convection stabilised medium. More specifically, the method comprises the steps to contact the sample with the separation medium; to apply a voltage across said medium; and to observe the results by analysis of one or more sections of the separation medium. In the present method, a disulphide-comprising compound is added before or during the procedure to make an excess of reactive disulphide groups accessible to react with the cysteine groups of the proteins and/or peptides all through the separation procedure. The present invention also relates to electrophoretic separation media that comprises reactive disulphide groups, such as polyacrylamide gels, and the use of a solution that comprises reactive disulphide groups to pretreat an electrophoretic separation medium.
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申请公布号 |
US2003221963(A1) |
申请公布日期 |
2003.12.04 |
申请号 |
US20020230526 |
申请日期 |
2002.08.29 |
申请人 |
BJELLQVIST BENGT;OLSSON INGMAR;PALMGREN RONNIE |
发明人 |
BJELLQVIST BENGT;OLSSON INGMAR;PALMGREN RONNIE |
分类号 |
B01D57/02;B03C5/00;C07K1/26;G01N27/447;(IPC1-7):G01N27/26 |
主分类号 |
B01D57/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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