NEW PRIMERS FOR THE DETECTION AND IDENTIFICATION OF BACTERIAL INDICATOR GROUPS AND VIRULENCE FACTORS

摘要

The present invention relates to new primers for the detection of any of three important bacterial indicator groups used in food microbiology and two virulence factors, which are associated with the aetiology of several types of watery and bloody human diarrhoea. It also provides a method for subtyping the two virulence factors. Furthermore, the present invention also relates to use of the primers, as well as use of the primers in a method which enables this detection, as well as the detection of new emerging pathogenic bacteria. The bacterial indicator group are identified by using primers specific for the aa (6') - Ii, eep, gadA and GadB genes. The virulence factors are identified by using primers specific for the stx and eae genes.