TETHERED ACTIVITY-BASED PROBES AND USES THEREOF

摘要

Methods and compositions are described for analyzing complex protein mixtures, such as proteomes, using activity-based probes. In particular, probes that specifically react with and bind to the active form of one or more target proteins are employed. Labeled proteins and/or peptides can be sequestered using a tABP covalently bound ("tethered") to a solid phase, and, upon cleavage of the tether, can be related to the identity, presence, amount, or activity of active members of the desired target protein class. The methods and compositions described herein can be used, for example, to provide diagnostic information concerning pathogenic states, in identifying proteins that may act as therapeutic targets, and in drug discovery.