METHODS FOR ISOLATING AND CHARACTERIZING SHORT-LIVED PROTEINS AND ARRAYS DERIVED THEREFROM

摘要

Compositions, kits and methods are provided for isolating and characterizing short−lived proteins. The method comprises: taking a library of cells, each cell in the library expressing a fusion protein comprising a reporter protein and a protein encoded by a sequence from a cDNA library derived from a sample of cells, the sequence from the cDNA library varying within the cell library; modifying a rate of protein expression or degradation by cells in the library; and selecting a population of cells from the library of cells based on the population of cells having different reporter signal intensities than other cells in the library, the difference being indicative of the population of cells expressing shorter lived fusion proteins than the fusion proteins expressed by the other cells in the library; and determining protein sequences of the fusion proteins of the selected population of cells. Also provided are oligonucleotide, protein and antibody arrays derived from short−lived proteins. The arrays can be used for efficiently profiling expression of short−lived proteins, screening for binding agents and comparing expression levels under different conditions.