发明名称 |
Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides |
摘要 |
Method of gene shuffling using oriented ligation, whereby at least two fragments are adjacently hybridized on an assembly template. Invention is particularly aimed at generating novel polynucleotides that differ in some advantageous respect compared to a reference sequence. Invention further includes sequences created by the method, hosts and vectors containing same, and proteins translated therefrom.
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申请公布号 |
US2003104417(A1) |
申请公布日期 |
2003.06.05 |
申请号 |
US20020131175 |
申请日期 |
2002.04.25 |
申请人 |
PROTEUS S.A. |
发明人 |
DUPRET DANIEL;MASSON JEAN MICHEL;LEFEVRE FABRICE |
分类号 |
C07K14/245;C12N15/10;(IPC1-7):C12Q1/68;C12P19/34;C12M1/34 |
主分类号 |
C07K14/245 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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