Bacillus strain and antibiotic screening method

摘要

A Bacillus strain has a chromosome with the following modifications: a mutation of a spoIIIE gene which blocks transfer of the prespore chromosome; a mutation which prevents loss of SpoOJ function from blocking sporulation; a first reporter gene dependent on sigmaF factor and placed at a location where impaired SpoOJ function leads to increased trapping in the prespore; and a second reporter gene having a promoter which is dependent on sigmaF factor and where impaired SpoOJ function leads to reduced trapping in the prespore. The strain is useful in a method of screening for putative antibiotics.