| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide a cell-culturing container attached with frozen anchorage dependant animal cells, capable of being used for an experiment using objective cells just after its thawing and not peeling off the cells after the thawing. SOLUTION: This cell-culturing container is provided by culturing the anchorage dependant animal cells on the surface of the culturing container, cooling within a 0-10 deg.C range, exchanging its medium with a medium for freezing and freezing. At the beginning of a culture, it is thawed at <=10 deg.C, its medium is changed with a medium for culturing and then the culturing is started.</p> |
